A Native Whole Blood Thrombin Generation Assay Allows Discrimination of Whole Blood Samples with FVIII Levels below 1%
نویسندگان
چکیده
منابع مشابه
Whole-blood thrombin generation monitored with a calibrated automated thrombogram-based assay.
BACKGROUND The calibrated automated thrombogram (CAT) assay in plasma is a versatile tool to investigate patients with hypo- or hypercoagulable phenotypes. The objective was to make this method applicable for whole blood measurements. METHODS Thin-layer technology and the use of a rhodamine 110-based thrombin substrate appear to be essential for a reliable thrombin generation (TG) assay in wh...
متن کاملThe long-awaited whole-blood thrombin generation test.
Thrombin is a multifunctional protease that, in addition to hemostasis, supports other “nonhemostatic” mechanisms, such as the regulation of vascular permeability, vascular tone, inflammation, and angiogenesis. There is also evidence that thrombin plays a role in the pathogenesis and progression of liver fibrosis (1 ). Among the hemostasis functions played by thrombin, one may list the fibrinog...
متن کاملDirect enzymatic assay for %HbA1c in human whole blood samples.
OBJECTIVES Development and validation of a direct enzymatic HbA1c assay that utilizes a single channel on chemistry auto-analyzers without the need to run separate glycated hemoglobin and total hemoglobin assays. DESIGN AND METHODS An enzyme based single channel assay was developed to measure %HbA1c in human whole blood samples. The performance characteristics of the Diazyme Direct Enzymatic ...
متن کاملAutologous thrombin: intraoperative production from whole blood.
Thrombin is routinely combined in surgical practice with a fibrinogen source to prepare fibrin sealant to promote hemostasis or with platelet concentrates to prepare platelet gels to enhance wound healing. The purpose of this study was to evaluate the robustness and reproducibility of a new sterile handheld disposable thrombin-processing device (TPD) to generate autologous human thrombin in the...
متن کاملHomogeneous immunoassay of whole-blood samples.
Proof of principle has been shown for a rapid, quantitative, homogeneous immunoassay capable of analyzing whole-blood samples. The assay was performed with test cards and a small instrument designed for use at the point of care. The immunoassay has an immunological "front end" combined with a coagulation cascade chemistry "back end" and is made possible by combining two patented technologies: (...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Blood
سال: 2014
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v124.21.4215.4215